By using the complementation test an unknown mutant can be rapidly assigned to a particular gene . 采用互補測驗可迅速地將某一未知突變型歸屬于某一特定基因。
Human elongator complex serves a role in chromatin remodeling through modifying the lysine residues of histone h3 and h4 during transcription elongation in higher cells . the main results and conclusions are as followings . 1 using depletion strain of yeast as material , we have established a system for complementation test and gene expression analysis of human elongator complex 4 、利用點突變和plasmidshuffle等技術構建了含h3k14一r 、 h4ks ~ r單突變和h3k14一側h4ks一r雙突變的e1p3乙菌株,通過功能互補實驗分析少凡p3對突變株的補償功能,證實酵母elongator可對h314k / h4sk進行乙酞化修飾。